SNRK modulates mTOR-autophagy pathway for liver lipid homeostasis in MAFLD
SNRK通过mTOR自噬通路调节MAFLD肝脏脂质稳态
作者单位：广东省人民医院 & 南方医科大学&中山大学第三附属医院
产品名称：细胞自噬PCR 阵列 Autophagy PCR Array
原文方法介绍：
...To investigate the regulation of autophagy by SNRK, we analyzed autophagy-related genes 167 using a mouse Autophagy PCR Array (including key genes of autophagy, such as ulk1, ulk2, 168 atg3, atg7, atg16l1, and beclin1) in whole liver extracts (Figure 4D). ...
...Additionally, we analyzed the expression of autophagy-related genes in the 216 whole liver extract using a mouse Autophagy PCR Array (Figure 5I). ...
...To determine differential gene expression associated with autophagy, cDNA was synthesized 483 from isolated RNA as mentioned above, and 40 ng of cDNA was added into each well of a 96- 484 well pre-manufactured mouse Autophagy Array (WCGENE, WC-MRNA0060-M). Real-time 485 PCR and data analyses were performed according to the manufacturer’s instructions 486 (WCGENE, WC-MRNA0060-M). Briefly, the expression level of each gene was obtained by 487 comparing it to internal controls on the same array. The relative expression levels of genes in 488 the experimental group compared with the control group were then calculated, setting control 489 samples as "1."...
