摘要

本研究的目的是研究微生物燃料电池（MFCs）在产甲烷菌活性有限的条件下，磺胺嘧啶（SDZ）的生物降解效率、耐药基因（ARGs）的发育和微生物群落的变化。结果表明，在MFCs和开路控制条件下，随着产甲烷菌的抑制，SDZ的去除性能下降。产甲烷菌的抑制作用使精氨酸的相对丰度增加。研究了SDZ的生物降解机理，即SDZ首先被分解为苯胺和嘧啶-2-基氨基磺酸，然后转化为小分子。土杆菌是MFCs中的优势微生物，具有降解SDZ的潜力。这些发现表明，通过抑制mfc中的产甲烷菌，发电与SDZ去除和ARG生成之间存在一种权衡。

The aim of this work was to study sulfadiazine (SDZ) biodegradation efficiency, antibiotic resistance genes (ARGs) development and shift of microbial communities under conditions of limited methanogens activity in Microbial fuel cells (MFCs). The results indicated that the removal performance of SDZ was decreased with the suppression of methanogens in both MFCs and open-circuit controls. The relative abundances of ARGs were even enhanced by the inhibition of methanogens. The biodegradation mechanism of SDZ was obtained, in which SDZ was initially divided into aniline and pyrimidin-2ylsulfamic acid, then converted into small molecules. Geobacter was found as the dominant microorganism, indicating its potential to degrade SDZ in the MFCs. These findings suggest there is a trade-off between electricity production and SDZ removal and ARG development by the mean of methanogen inhibition in MFCs.