摘要

在与庆大霉素和洛伐他汀发酵残渣（GFR和LFR）共堆肥的过程中，评估了细菌群落组成的动态，以及13个抗生素抗性基因（ARGs）和8个可移动遗传元件（MGEs）。利用下一代测序，确定了与堆肥不同阶段相关的关键细菌类群。最重要的是，芽孢杆菌属于厚壁菌门，与庆大霉素的降解、有机物（OM）和溶解有机碳（DOC）的分解以及堆肥循环的高温阶段的延长有关。在堆肥过程中，不同ARGs/MGEs的模式不同。然而，总拷贝数和标准化拷贝数（细菌数）都很高。各种ARGs的丰度与细菌的丰度和群落组成有关，单个ARGs的变化规律受MGEs和细菌选择性的影响。

Dynamics in bacterial community composition, along with 13 antibiotic resistance genes (ARGs) and eight mobile genetic elements (MGEs), were assessed during co-composting with gentamicin and lovastatin fermentation residue (GFR and LFR, respectively). Using next generation sequencing, the key bacterial taxa associated with the different stages of composting were identified. Most importantly, Bacillus, belonging to Phylum Firmicutes, was associated with enhanced degradation of gentamicin, decomposition of organic matter (OM) and dissolved organic carbon (DOC), and also extension of the thermophilic phase of the composting cycle. During the course of composting, the patterns of different ARGs/MGEs varied. However, the total and the normalized (to bacterial numbers) copies both remained high. The abundance of various ARGs was related to bacterial abundance and community composition, and the changing pattern of individual ARGs was influenced by the selectivity of MGEs and bacteria.

https://www.sciencedirect.com/science/article/abs/pii/S096085241830511X