镁浸渍膜通过 MagT1 对富含半胱氨酸的酸性分泌蛋白(SPARC)进行 N-连接糖基化，促进大鼠颅骨缺损处的骨再生
原文方法介绍：
We also verified the adhesion-related mRNA expression by PCR array, in which 55 genes were upregulated more than twofold, and 18 of these were significant when more magnesium was supplemented to MC3T3 with silenced MagT1... added to PCR array (WcGene Biogene, WC-MRNA0057-M),...
本研究通过PCR Array同时检测多个基因在不同样本（有镁浸渍膜处理的大鼠颅骨缺损组织和未处理的对照组织）中的表达情况。通过比较基因表达水平，可发现哪些基因在镁浸渍膜促进骨再生过程中发生了上调（表达增加）或下调（表达减少）。例如，对于与骨再生相关的基因，如成骨细胞标志物基因（如碱性磷酸酶基因 ALP 等）、细胞外基质蛋白基因（如胶原蛋白基因等）以及信号通路相关基因，PCR Array 能够快速地确定它们的表达变化。
文章来源：
Chen W, Lu H, Yu W, Huang L, Bian M, Wang N, Xiang X, Mo G, Zhang C, Li Y, Jiang L, Zhang J. Magnesium-Impregnated Membrane Promotes Bone Regeneration in Rat Skull Defect by N-Linked Glycosylation of SPARC via MagT1. Adv Healthc Mater. 2024 Dec 4:e2402705. doi: 10.1002/adhm.202402705. Epub ahead of print. PMID: 39632347.
