沃吉PCRArray应用：ArthritisRheumato(IF11.4)发表蛋白质组+基因表达谱发现TGFb在PVNS的作用

Mapping and analysis of protein and gene profiles identify the important role of TGF-β

in synovial invasion in pigmented villonodular synovitis

蛋白质及基因图谱的定位、鉴定与分析，对色素沉着绒毛结节性滑膜炎患者滑膜侵袭情况中

转化生长因子β起重要作用

原文方法介绍：

...RNA quality was determined using a spectrophotometer, and RNA was reverse transcribed into cDNA using a Wcgene mRNA cDNA kit. The cDNA was analyzed with an EMT Pathway PCR Array (Wcgene Biotech, China).

对于研究色素沉着绒毛结节性滑膜炎患者中与滑膜侵袭相关、受转化生长因子β调控的基因情况时，本研究通过PCR Array同时检测多个特定的目的基因（比如那些已知或推测与滑膜细胞增殖、迁移、细胞外基质重塑等和滑膜侵袭密切相关且受转化生长因子β影响的基因）。快速筛选出在患病状态下表达有差异的基因，有助于聚焦后续深入研究的关键基因靶点......

文章来源：

Li T,Xiong Y,Li J,Tang X,Zhong Y,Tang Z,Zhang Q,Luo Y.Mapping and Analysis of Protein and Gene Profile Identification of the Important Role of Transforming Growth Factor Beta in Synovial Invasion in Patients With Pigmented Villonodular Synovitis.Arthritis Rheumatol. 2024 Nov;76(11):1679-1695.doi:10.1002/art.42946.Epub 2024 Aug 29. PMID: 38973550.